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The most common examples are the α-helix, β-sheet and turns. Antibody or antigen/antibody complex (IP) is first captured on the magnetic beads. So if your needs are 2,000 calories, that’s 200–700 calories from protein, or 50–175 grams. The multipoint attachment of protein A to Sepharose ® results in very low ligand leakage over a wide range of elution conditions. The various purification options for Protein A Sepharose ® chromatography media are summarized in Table 3.
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